Evaluation of Safety and Potency of Kyasanur Forest Disease (KFD) Vaccine Inactivated with Different Concentrations of Formalin and Comparative Evaluation of In Vitro and In Vivo Methods of Virus Titration in KFD Vaccine

We evaluated the safety and potency of Kyasanur Forest disease (KFD) vaccine inactivated with different formalin concentrations in mice, since side effects due to higher have been a major reason for refusal. Furthermore, an objective reduce use mice testing, we performed quantification KFD virus by real-time PCR compared it vivo titration mice. The prepared chicken embryo fibroblast cells was 0.04%, 0.06%, 0.08% formalin. 0.04% 0.06% failed test, whereas safe potent log protective index 5678 This reduced content may induce no/lesser side-effects pain/swelling which increase acceptance. on individual harvests interpreted that when CT value each harvest is <20, will sufficient viral particles pass test. Comparison tenfold dilutions pooled inoculation test revealed 1MLD50 lies dilution yields values between 31 34.